Composite
Part:BBa_K310006:Design
Designed by: Erin Borchardt Group: iGEM10_UIUC-Illinois (2010-09-24)
ArsR under the control of J23100, includes RBS B0034 and terminator B0015
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 199
Design Notes
Site directed mutagenesis was performed in the coding region of ArsR due to the presence of XbaI site. This changed the coding sequence AGATCT to AGGTCT.
Source
I used PCR to isolate ArsR from E. coli K-12 MG1655 genome and attached B0015, B0034 and J23100 from the parts registry.
References
This paper was particularly helpful when working with the Ars Operon and Arsenic parts within E. coli: [http://aem.asm.org/cgi/reprint/70/8/4582 Enhanced Arsenic Accumulation in Engineered Bacterial Cells Expressing ArsR]